FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_47.35100000045521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_47.35100000045521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291641
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC4364614.965659835208356RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT197746.780253805191999No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG19950.6840601972973622No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC16620.569878720755998No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC15570.5338755524771894No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA14360.49238618712732435No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA13350.45775456811627996No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT8340.28596802232882207No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5900.20230351699520988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA5630.19304555943780197No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA5570.19098823553615576No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5080.1741867570060451No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC5080.1741867570060451No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC5000.17144365847051685No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4950.16972922188581166No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4860.16664323603334238No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4000.13715492677641347No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA3730.12789696921900556No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3200.10972394142113077No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3160.10835239215336663No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC2980.10218042044842802No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2970.10183753313148701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC49000.043.34578345
GCCGTCT1800.039.99893645
ACTATCG250.00210481235.9990411
TGCAGTC250.00210481235.9990445
ATGCCGT69150.030.68247844
GTTCCAT300.00513308630.0094931
GAACACG300.005137393730.0043456
CCTACAC1650.030.0043433
TCGGTCA300.005141704329.999215
TATGCCG71600.029.6325943
GTGGACA71850.029.62342831
CGTATGC71950.029.61352541
TGTGGAC72100.029.58312230
GGACATC71700.029.55988133
ACGAGAC72250.029.55284722
CCTGTGG72350.029.54309528
TCGTATG72050.029.54119940
CATCTCG71750.029.53928436
ATCTCGT71850.029.52948637
GACATCT71700.029.52850234
Produced by FastQC (version 0.11.2)