FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_46.35100000045507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_46.35100000045507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330379
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC217396.580018705789412RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT43621.320301835165068No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10760.3256865599811126No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10430.3156980316545543No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC10000.3026826765623723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA9390.2842190332920676No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC7210.21823420980147043No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC7030.21278592162334775No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG6460.1955330090592925No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG6380.19311154764679353No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA6040.18282033664367286No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6020.18221497129054812No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5190.15709230913587124No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT5070.15346011701712275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG4690.14195817530775262No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4680.14165549263119023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.1259159934499469No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4030.12198111865463603No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3800.11501941709370148No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3780.11441405174057674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG3550.10745235017964216No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT3540.10714966750307979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23000.042.5540645
CGGTCGA301.13820686E-437.4997510
ACCGCTA250.0021050135.9997624
CGCATGT250.0021050135.9997636
GCTGCAT250.0021050135.999768
CTTAGCG250.0021050135.9997617
TACACCG250.0021050135.999765
ATGCCGT28100.034.83072744
CATCTCG28800.034.0622736
ACGAGAC29500.033.86417822
CGAGACC29400.033.826323
CCTGTGG29100.033.78843328
GAGACCT29450.033.7688724
TATGCCG29000.033.74977543
GGACATC29000.033.74977533
CACGAGA29600.033.7497721
TGTGGAC29100.033.71111330
CGTATGC29200.033.67271841
TGGACAT29200.033.67271832
TCGTATG29250.033.61515840
Produced by FastQC (version 0.11.2)