FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_45.351000000454ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_45.351000000454ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349895
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC75322.152645793738121RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT33260.9505708855513797No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG5710.16319181468726332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7700.042.95372845
TAGCCTA401.5561569E-533.754184
ACGTAGC352.8143474E-432.146841
CGGGCAC352.8167095E-432.14224234
ATGCCGT10600.030.98997744
CGTAGCC453.4810328E-530.0037172
GCCTAGA453.4810328E-530.0037176
TATAAAA453.4810328E-530.0037172
ATAGGGC300.005142902529.99942841
TATGCCG11000.029.8630743
GTATGCC11100.029.5940342
CGTATGC11350.029.14041541
CGAGACC11650.028.58314723
CTCGTAT11500.028.56467439
TCGTATG11600.028.5123940
ACCTGTG11350.028.34747927
GGCGGTA406.15819E-428.12446440
ATCGGGC406.15819E-428.12446436
GGACATC11700.028.0763933
TGGACAT11650.028.00375732