Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_45.351000000454ec.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 349895 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC | 7532 | 2.152645793738121 | RNA PCR Primer, Index 20 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT | 3326 | 0.9505708855513797 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG | 571 | 0.16319181468726332 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 770 | 0.0 | 42.953728 | 45 |
TAGCCTA | 40 | 1.5561569E-5 | 33.75418 | 4 |
ACGTAGC | 35 | 2.8143474E-4 | 32.14684 | 1 |
CGGGCAC | 35 | 2.8167095E-4 | 32.142242 | 34 |
ATGCCGT | 1060 | 0.0 | 30.989977 | 44 |
CGTAGCC | 45 | 3.4810328E-5 | 30.003717 | 2 |
GCCTAGA | 45 | 3.4810328E-5 | 30.003717 | 6 |
TATAAAA | 45 | 3.4810328E-5 | 30.003717 | 2 |
ATAGGGC | 30 | 0.0051429025 | 29.999428 | 41 |
TATGCCG | 1100 | 0.0 | 29.86307 | 43 |
GTATGCC | 1110 | 0.0 | 29.59403 | 42 |
CGTATGC | 1135 | 0.0 | 29.140415 | 41 |
CGAGACC | 1165 | 0.0 | 28.583147 | 23 |
CTCGTAT | 1150 | 0.0 | 28.564674 | 39 |
TCGTATG | 1160 | 0.0 | 28.51239 | 40 |
ACCTGTG | 1135 | 0.0 | 28.347479 | 27 |
GGCGGTA | 40 | 6.15819E-4 | 28.124464 | 40 |
ATCGGGC | 40 | 6.15819E-4 | 28.124464 | 36 |
GGACATC | 1170 | 0.0 | 28.07639 | 33 |
TGGACAT | 1165 | 0.0 | 28.003757 | 32 |