H23LNBCXX_l02n01_bir_0113_pc_44.351000000454c2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pc_44.351000000454c2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367756
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC	8873	2.412741056570117	RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT	1932	0.5253483287832149	No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA	837	0.22759655858775926	No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT	783	0.2129129096466135	No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG	710	0.19306279163358314	No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA	469	0.12753021024809927	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	431	0.11719727210433005	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	387	0.10523281741154462	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	985	0.0	42.7138	45
TCGCGCG	30	1.1387168E-4	37.498302	9
GGGGCTA	30	1.1387168E-4	37.498302	36
ATGCCGT	1155	0.0	36.232124	44
CGCGGAT	25	0.0021057178	35.99837	41
GGTCCGC	25	0.0021057178	35.99837	10
GTCCGCC	25	0.0021057178	35.99837	11
TGTGGAC	1205	0.0	35.47557	30
CATCTCG	1190	0.0	35.355537	36
TATGCCG	1195	0.0	35.20761	43
GGACATC	1215	0.0	35.18359	33
GTATGCC	1185	0.0	35.124992	42
CGTATGC	1210	0.0	34.957092	41
TGGACAT	1230	0.0	34.754524	32
ACCTGTG	1230	0.0	34.754524	27
CGAGACC	1225	0.0	34.71271	23
ACGAGAC	1225	0.0	34.71271	22
CTCGTAT	1225	0.0	34.52905	39
GTGGACA	1240	0.0	34.474243	31
CTGTGGA	1245	0.0	34.335793	29