FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_43.351000000454a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_43.351000000454a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318351
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC47011.4766719752725765RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT5290.16616878853843714No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3390.10648623688947105No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT250.002102014136.0098046
ATGCCGT5700.035.5248344
TATGCCG5900.034.32059543
GTATGCC5900.034.32059542
CGTATGC5950.034.03218541
TGCCGTC5950.034.03218545
GCCCACG6150.032.9254518
ACCTGTG6150.032.9254527
CACGAGA6150.032.9254521
CCCACGA6150.032.9254519
TGTGGAC6350.032.59705730
TGGACAT6250.032.3986432
CTGTGGA6350.032.24274429
GGAGTCC352.8165206E-432.141518
TCGTATG6300.032.1415140
CTCGTAT6350.031.88842839
GGACATC6350.031.88842833
CATCTCG6400.031.63930136
CGAGACC6400.031.63930123
ACGAGAC6450.031.39403322
Produced by FastQC (version 0.11.2)