FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_42.3510000004548f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_42.3510000004548f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303556
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC40441.3322088840279882RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT13360.4401164859202256No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3460.11398226356916022No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG3340.1100291214800564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4400.042.4396145
ATGCCGT5900.031.26855544
TATGCCG6050.030.493343
TTCGCTT300.005126926630.0172524
CGTATGC6250.029.87748541
CTCGTAT6300.029.64036239
GTATGCC6250.029.51751542
CGTTACT1150.029.3647042
CCGTTAC1150.029.3647041
CGAGACC6400.029.1772323
ACGAGAC6500.029.07447622
TCGTATG6550.028.85253340
TGTGGAC6350.028.69837230
CCCACGA6600.028.63395519
GGACATC6400.028.47416533
CACGAGA6700.028.20658321
ACGGGCA406.158642E-428.12263317
TCTCGTA6400.028.12263338
CGGGCAG406.158642E-428.12263318
ACCCCCG406.158642E-428.12263321
Produced by FastQC (version 0.11.2)