FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_41.35100000045465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_41.35100000045465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155786
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC1942412.46838611942023RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT52813.3899066668378413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG8520.5469040863748988No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT4360.2798711052341032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21850.043.352445
AATGCCA250.002101532436.010
ATGCCGT26800.035.3451544
GCCCACG27750.034.4594618
GAGCCCA27900.034.43548216
ACGAGAC27850.034.41651522
CGAGCCC28050.034.41176215
CCCACGA27800.034.3974819
CGAGACC27950.034.3738823
TCCGAGC28150.034.3694513
CCGAGCC28150.034.3694514
AGCCCAC27900.034.35483617
CACGAGA27900.034.35483621
CCACGAG27950.034.29337720
TATGCCG27700.034.2779843
CGTATGC27900.034.19354641
ACCTGTG27950.034.13237827
CCTGTGG27950.034.13237828
CATCTCG28100.034.11031736
CTCGTAT28050.034.09090839
Produced by FastQC (version 0.11.2)