FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_40.3510000004544c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_40.3510000004544c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239348
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC10740.44871902000434516No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT4500.18801076257165297No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4150.1733877032605244No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA3760.15709343717098118No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT3560.14873740327890772No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG2560.10695723381854037No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA352.8008144E-432.16811
CGCGCGC352.8145954E-432.14121610
TCGCGCG352.8145954E-432.1412169
TGCCGTC1900.030.78790345
GCCCACG1850.030.4038518
TCTAGAC300.005129997630.011013
GTATGCC1950.029.99846642
ATGCCGT1950.029.99846644
CGTATGC2000.029.24850541
TATGCCG2050.028.5351343
CTCGTAT2100.027.8557239
TCGTATG2100.027.8557240
CGAGACA2100.027.8557223
AGACAGG2150.027.20791425
CGCGCTT507.1376206E-526.99862112
CCCACGA2100.026.78434619
GACAGGA2200.026.5895526
GAGACAG2350.025.84974524
CACGAGA2200.025.56687521
TCTCGTA2300.025.43348538
Produced by FastQC (version 0.11.2)