FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_4.35100000044fca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_4.35100000044fca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353319
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC49661.4055287148440927No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT9080.25699155720467903RNA PCR Primer, Index 44 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6470.18312063602580103No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6040.17095033100399357No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA5660.16019517772890787No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT3770.10670244170282378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5500.041.3177945
GGGCGGA250.00210523535.9996645
CCCCCCT250.00210523535.9996644
GTATGCC6600.034.4314942
ATGCCGT6600.034.4314944
TATGCCG6650.034.17260743
CGTATGC6850.033.50333441
GAGACCC6900.033.2605624
TATCTCG6850.033.1748736
TCGTATG6850.033.1748740
CGAGACC7000.032.78540423
CACGAGA7050.032.55288721
TAGAATA6950.032.37379531
ACCCTAG6950.032.37379527
CCCACGA7100.032.3236419
GCCCACG7150.032.097618
ACGAGAC7150.032.097622
CCCTAGA7050.031.91459528
CTCGTAT7150.031.78291739
TCTCGTA7250.031.65487538
Produced by FastQC (version 0.11.2)