FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_39.35100000045422.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_39.35100000045422.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340142
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC105343.0969418654561918No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT18320.538598585296729No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG14030.4124747899406718No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG8270.24313375002204962No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA6640.19521258768396726No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4830.14199951784842801No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG4500.13229768743642362No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4360.12818175938284598No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT4060.11936191355375109No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4000.11759794438793211No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3850.11318802147338464No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA3740.1099540780027165No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3440.10113423217362162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11600.042.283145
ATGCCGT13350.036.74037644
TATGCCG14350.034.18006543
GTATGCC14400.034.06138642
CGTATGC14550.034.0195141
TCGTATG15000.033.1489240
CTCGTAT15100.032.92938639
GGATGTG15100.032.92938630
ATCTCGT15250.032.6054937
GATCTCG15400.032.43400636
GTGATCT15400.032.28790734
CAGGATG15500.032.22475428
ACAGGAT15550.032.12113627
TCTCGTA15500.032.07959738
GACAGGA15700.031.95755626
AGACAGG15750.031.85610225
GATGTGA15700.031.6709431
CGAGACA15850.031.65511923
AGGATGT15800.031.6128929
TGTGATC15700.031.52763433
Produced by FastQC (version 0.11.2)