FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_38.35100000045408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_38.35100000045408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28100
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC304610.839857651245552No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT6992.487544483985765No Hit
TTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTCT1340.47686832740213525No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA1060.37722419928825623No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT890.3167259786476868No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA780.2775800711743772No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA740.2633451957295374No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT690.24555160142348756No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT680.24199288256227758No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG670.23843416370106763No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT660.23487544483985767No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC590.20996441281138792No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA500.1779359430604982No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT490.17437722419928825No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC490.17437722419928825No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC490.17437722419928825No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA420.1494661921708185No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT410.14590747330960854No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC380.13523131672597866No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA370.1316725978647687No Hit
ATACGAGATCACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAG350.12455516014234876No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA340.12099644128113879No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC340.12099644128113879No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT300.10676156583629894No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT290.10320284697508895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT206.916324E-445.00000445
TGCCGTC3700.042.56756645
ATGCCGT4800.032.81250444
CGGGCAG352.7514133E-432.1428574
GATCTCG5000.031.0536
GTATGCC5100.030.88235342
CGTATGC5100.030.88235341
ATCTCGT5050.030.74257537
TGATCTC5050.030.74257535
TCGTATG5050.030.74257540
TATGCCG5150.030.58252343
ATGTGAT5150.030.58252332
CTCGTAT5150.030.58252339
TGTGATC5150.030.58252333
GTGATCT5100.030.44117734
AGGATGT5200.030.28846429
CAGGATG5200.030.28846428
TCTCGTA5150.030.1456338
GGATGTG5300.030.1415130
GATGTGA5350.029.85981631
Produced by FastQC (version 0.11.2)