FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_36.351000000453c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_36.351000000453c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383872
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC74421.9386670556852286No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT19910.518662470823608No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG12400.32302434144714903No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT8530.2222094864954985No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC7100.18495748582860952No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT7020.18287345781927308No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA6470.16854576525508502No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT5690.1482264921640547No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA5560.14483994664888294No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT4610.12009211403801268No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT4590.11957110703567857No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC4540.11826858952984327No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4470.1164450650216739No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4150.10810895298432811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9450.042.1412745
CGGGTCA250.00210575335.99864616
ATGCCGT12050.033.0485544
AAAAAGT14850.031.3788051
AAAAGTA15400.031.2732052
TATGCCG13050.030.51609443
CGTATGC13500.030.16553141
GTATGCC13400.030.0548442
CCATCGC300.005137434730.006693
TCGTATG13550.029.88817440
CTCGTAT13500.029.49888839
CGAGACA13850.029.0783323
GATCTCG13700.029.06824936
TCTCGTA13800.028.8576138
GGATGTG13900.028.81186530
CACGAGA14000.028.76677521
GTGATCT13900.028.6534
GACAGGA14100.028.56275626
AGACAGG14100.028.56275625
ACGAGAC14100.028.56275622
Produced by FastQC (version 0.11.2)