FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_35.3510000004539d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_35.3510000004539d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288685
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC44221.5317733862168108No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT9050.3134904827060637No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3460.11985381990751165No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG3000.10391949702963438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4950.038.17917345
GAGCACC250.002105216735.99750522
ATGCCGT5950.031.38437844
GCCGTCT602.4098335E-729.9979245
TATGCCG6250.029.8779343
CGTATGC6300.029.64080241
GTATGCC6300.029.64080242
ATCTCGT6550.028.50947637
CTCGTAT6600.028.29349139
GTATAGT406.132447E-428.1425511
CACGAGA6650.028.08076321
TCGTATG6700.027.87120440
CGAGACA6650.027.7424423
TCTCGTA6750.027.6647538
GGATGTG6550.027.47901230
GATCTCG6650.027.40411836
GACAGGA6600.027.27083626
ACGAGAC6850.027.26088522
CCACGAG6900.027.06334120
AGTACTA507.1182825E-527.0121675
Produced by FastQC (version 0.11.2)