FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_33.35100000045366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_33.35100000045366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137463
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC84146.120919811149182No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT16511.2010504644886262No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG6010.4372085579392273No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1550.11275761477633982No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.10912027236419983No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.10402799298720383No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCT207.011885E-444.99963828
CGTTCAA207.011885E-444.99963812
TGCACGC207.011885E-444.99963827
TGCCGTC8350.043.92180345
TGGACTC301.13475515E-437.49969535
ATGCCGT9750.037.38431544
TCGTTCA250.00210075835.99970611
GGACTCA250.00210075835.99970636
CTCCCAT250.00210075835.99970630
TCTCCCA250.00210075835.99970629
CGTATGC10300.035.6065141
CTCGTAT10350.035.43449839
TATGCCG10300.035.38806543
CGAGACA10450.035.3107223
ATCTCGT10350.035.21710637
GTATGCC10350.035.21710642
GGATGTG10300.035.1696230
GATCTCG10300.035.1696236
TCTCGTA10450.035.0954138
TCGTATG10450.035.0954140