FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_32.3510000004534d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_32.3510000004534d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239129
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC39441.6493189868230118No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT11310.47296647416248133No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2780.11625524298600337No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA2630.10998247807668664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4650.039.6761345
GCCGTCT551.1216798E-732.72620845
ATGCCGT6000.030.74899944
CACGAGA6350.030.47144921
CATCTCG6300.030.35615536
CCCACGA6400.030.2333919
TATGCCG6200.030.1199943
CTATACT300.005134765530.00534
ATACTGG300.005134765530.00536
ATTCGGT300.00514002129.99902543
CACGTTA300.00514002129.99902530
GTATGCC6150.029.99902342
GAGACCA6550.029.54102324
CGAGACC6550.029.54102323
GCCCACG6650.029.43513318
CTCGTAT6500.029.4221239
GACCATC6600.029.3172326
ACGAGAC6600.029.3172322
ATCTCGT6600.028.97633237
CAGACAT6600.028.97633232
Produced by FastQC (version 0.11.2)