FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_31.35100000045323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_31.35100000045323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297781
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC4256914.29540501240845No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT177545.962099663846921No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG23600.7925287375621681No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC11210.37645115034202986No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8670.29115356587559316No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT8430.2830939515952999No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA8120.27268361648325445No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA7790.26160164684785125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA5760.19343074272703764No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5480.18402785940002886No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4970.16690117905440574No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4920.16522209274601132No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4140.1390283463350583No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3720.12492402134454517No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3370.11317041718578419No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA3310.11115551361571088No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3290.1104838790923531No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC3130.10511080290549095No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC3090.10376753385877541No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT2980.10007354398030768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC49800.043.28119745
CGACTAG458.6741966E-734.99843217
TTCGACT458.6741966E-734.99843215
GCCGTCT1800.033.7484945
GTGCCCT352.8161323E-432.1414230
ATGCCGT67800.031.79061144
CTAGTTA501.9757972E-631.49858920
TCGACTA501.9757972E-631.49858916
TATGCCG70350.030.67026943
GTATGCC70550.030.58332342
CGTATGC70800.030.53888541
ATCTCGT70850.030.48557937
CTCGTAT71050.030.46309939
TCTCGTA71000.030.45286238
CATCTCG70650.030.44449636
GACATCT70850.030.35855534
TCGTATG71450.030.29255540
CAGACAT71050.030.20976632
AGACATC71100.030.1568833
ACGAGAC72400.030.01937522
Produced by FastQC (version 0.11.2)