FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_30.35100000045309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_30.35100000045309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278394
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC104523.7543912584322934No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT26320.9454226743392459No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4270.1533797423795053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11350.042.4198645
ATGCCGT14100.034.1464844
CGTATGC14700.032.90579641
TATGCCG14650.032.86453243
GTATGCC14650.032.86453242
TCGTATG14750.032.79425440
CTCGTAT14900.032.46410839
CATCTCG15000.032.3976736
CAGACAT14800.032.3794332
ACGAGAC15050.032.2900422
GACATCT14900.032.16211734
AGACATC15100.031.88512633
TCTCGTA15250.031.86656238
ATCAGAC15100.031.58713530
CACGAGA15400.031.55617321
CCCACGA15350.031.51239419
GACCATC15350.031.51239426
CCACGAG15350.031.51239420
TCAGACA15150.031.33438131
CGAGACC15550.031.25177423
Produced by FastQC (version 0.11.2)