FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_3.35100000044f94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_3.35100000044f94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34700
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC991028.55907780979827No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT7442.144092219020173RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3511.0115273775216138RNA PCR Primer, Index 44 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG2330.6714697406340058No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC2110.6080691642651297RNA PCR Primer, Index 37 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT930.26801152737752165RNA PCR Primer, Index 37 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC451.8373612E-840.045
TGCCGTC11950.039.35146745
CCGAGCC13100.037.61450214
TCCGAGC13150.037.47148513
AGCCCAC13050.037.4137917
ATGCCGT12600.037.3214344
GAGCCCA13100.037.2709916
GCCCACG13050.037.2413818
CGAGCCC13250.037.1886815
CTCCGAG13150.037.1292812
TCTCCGA13250.036.84905611
CCCACGA13200.036.8181819
ACGAGAC13150.036.78707522
CACGAGA13200.036.64772821
GAGACCC13200.036.64772824
TCTCGTA13100.036.58396538
TATCTCG13050.036.55172336
CCACGAG13300.036.5413520
GACCCTA13250.036.50943426
AGACCCT13250.036.50943425
Produced by FastQC (version 0.11.2)