FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_29.351000000452ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_29.351000000452ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342519
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC183705.363206128711108No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT46901.3692671063503046No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG10970.3202742037667983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC20300.043.778845
TAATAGG250.00210250136.008882
GCGAGCG250.002105517335.99836721
ATGCCGT25000.035.36839744
GTATTAG458.639727E-735.018861
TATGCCG25850.034.2054143
CATCTCG25900.034.13937836
CTCGTAT26000.034.0946139
GTATGCC26000.034.0080742
TCGTATG26200.033.83434340
CGTATGC26150.033.81299641
TCTCGTA26150.033.81299638
CAGACAT26300.033.7912432
ATCAGAC26250.033.59847630
TCAGACA26500.033.53621331
GACATCT26450.033.51454534
AGACATC26450.033.4294833
ATCTCGT26600.033.2409737
CGAGACC26650.033.17860823
CCCACGA26800.033.07685519
Produced by FastQC (version 0.11.2)