FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_28.351000000452c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_28.351000000452c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348683
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC112063.213807383784125No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT25600.7341912281355845No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4820.13823444217240302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11600.043.44647645
ATGCCGT14250.035.36695544
TATGCCG14900.033.824143
GTATGCC14900.033.824142
CGTATGC15000.033.748641
CATCTCG15200.033.008536
TCGTATG15350.032.97908840
CCCACGA15550.032.6996119
CTCGTAT15600.032.59480339
GACATCT15200.032.5644434
CAGACAT15200.032.5644432
GCCCACG15650.032.49066518
TCTCGTA15750.032.42722738
CCACGAG15800.032.3246120
ACGAGAC15850.032.2226422
ATCAGAC15700.031.95727730
CACGAGA16000.031.9205521
GACCATC16000.031.6393126
CGAGACC16150.031.62407723
ATCTCGT15950.031.59743937
Produced by FastQC (version 0.11.2)