FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_26.35100000045281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_26.35100000045281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316782
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC99893.153272597559205No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT30720.9697520692463588No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4820.15215510982315913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8900.041.96614545
CTAAATG250.002104845835.9998744
GCCGTCT352.8158538E-432.14274645
ATGCCGT12300.030.36574744
GTATTAT300.005137815630.0046331
CCCCTTA300.00514178429.99989510
GTGGGAC453.4830322E-529.99989517
TAAACGC300.00514178429.99989515
GTTAAAC300.00514178429.9998953
AATTCCG300.00514178429.9998955
TATGCCG13000.028.73066743
GTATGCC13050.028.6205942
CGTATGC13200.028.29535541
CATCTCG13350.028.14596736
GTATAGT406.1506487E-428.1293431
TAATAGC406.156332E-428.1249034
CCTTAAA406.156332E-428.12490312
TGGACTA406.156332E-428.1249035
CATAGGG406.156332E-428.12490329
TTAAACG406.156332E-428.12490314
Produced by FastQC (version 0.11.2)