FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_25.35100000045267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_25.35100000045267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123188
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC1598512.976101568334578No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT28782.336266519466182No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG4930.400201318310225No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT3860.3133422086566873No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC1570.12744747865051792Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG207.010309E-444.9979733
GTTTCGT207.010309E-444.9979734
TTACGTG207.010309E-444.997977
TGCCGTC16850.042.32746545
TTTAGCC356.204895E-638.56968727
TTAGCCT356.204895E-638.56968728
ATGGCAC250.00210029235.9983758
CGTGATA250.00210029235.99837510
GGGAGAG250.00210029235.99837521
CACTCCT250.00210029235.99837511
GGTTGGG250.00210029235.9983758
TCATACG250.00210029235.99837538
CCTTGTA250.00210029235.99837515
ATGCCGT20000.035.66089244
CCCACGA20550.035.03491619
GACCATC20450.034.98619526
CGTATGC20600.034.9498841
TCGTATG20600.034.9498840
CCACGAG20600.034.9498820
CTCGTAT20550.034.92543439
Produced by FastQC (version 0.11.2)