FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_24.3510000004524e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_24.3510000004524e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49331
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC3470.7034116478482091RNA PCR Primer, Index 23 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTGA206.9677166E-445.00000417
CGTATGC502.0008883E-1145.041
CTCGTAT502.0008883E-1145.039
TATGCCG502.0008883E-1145.043
CCGAGCC502.0008883E-1145.014
TCGTATG502.0008883E-1145.040
CGAGCCC502.0008883E-1145.015
CGAGACG502.0008883E-1145.023
ATGCCGT502.0008883E-1145.044
CTCCGAG555.820766E-1140.90909212
GAGAGTT555.820766E-1140.90909229
AGACGAG555.820766E-1140.90909225
GAGACGA555.820766E-1140.90909224
GTATGCC555.820766E-1140.90909242
GAGCCCA555.820766E-1140.90909216
CCACGAG555.820766E-1140.90909220
TGCCGTC555.820766E-1140.90909245
TGATCTC659.094947E-1238.0769235
GCCCACG601.4733814E-1037.49999618
TCCGAGC601.4733814E-1037.49999613
Produced by FastQC (version 0.11.2)