FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_23.35100000045224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_23.35100000045224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203219
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC128956.345371249735507RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT24341.1977226538857095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG6600.32477278207254245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGC207.0195686E-445.00000416
TGCCGTC13900.042.73381445
ATGCCGT16100.036.89441344
CGTATGC17000.035.20588341
TCGTATG17050.035.1026440
TATGCCG16950.035.04424743
CTCGTAT17150.035.02915639
GTATGCC17000.034.94117742
GATCTCG16950.034.91150336
CGAGACG17150.034.76676623
ACGAGAC17300.034.72543322
TCTCGTA17300.034.5953838
CCACGAG17500.034.3285720
ATCTCGT17400.034.26724237
ACGAGAG17400.034.26724227
GCCCACG17500.034.218
CCCACGA17500.034.219
GACGAGA17500.034.226
CACGAGA17600.034.0056821
CGAGAGT17550.033.97435828
Produced by FastQC (version 0.11.2)