FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_22.3510000004520a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_22.3510000004520a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108047
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC19751.8279082251242516RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT3790.35077327459346397No Hit
ACATGGTACCGTACGGAAGATTTAGATAAGAAAGAAGTAACACACTGACGA1610.14900922746582504No Hit
CCATATGACTTAGCCATTTTCAGTTTGGGATATTGTTATCCAAAGAACCAA1590.14715818116190177No Hit
CATTAAAACCGGAAACACACAAACCAATGACTTATTCGAGAAAACACTCAT1460.13512638018640036No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT1360.12587114866678392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.043.99728445
CGTATGC3000.032.99796341
GTATGCC3000.032.99796342
ATGCCGT3000.032.99796344
CGAGACG2950.032.79458623
TATGCCG3050.032.45701243
TCGTATG3050.032.45701240
CCACGAG3050.032.45701220
CGAGAGT3000.032.2480128
CACGAGA3100.031.93351421
CCCACGA3100.031.93351419
GATCTCG3100.031.93351436
CTCGTAT3150.031.42663239
AGTTGAT3150.031.42663232
ACGAGAC3100.031.20775222
GTTGATC3200.030.9355933
GCCCACG3200.030.9355918
TCTCGTA3200.030.9355938
ATCTCGT3250.030.4596637
AGAGTTG3250.030.4596630
Produced by FastQC (version 0.11.2)