FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_21.351000000451ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_21.351000000451ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245391
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC66992.7299289705001404RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT22550.9189416074754166No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG4010.16341267609651536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8900.043.48098445
ATGCCGT11750.032.93453244
TCGTATG12200.031.9041540
TATGCCG12150.031.85026643
GTATGCC12150.031.85026642
CGAGAGT12100.031.79593728
CGTATGC12250.031.77392841
CGAGACG12250.031.77392823
ACGAGAC12400.031.38956622
GATCTCG12350.031.3344736
GCCCACG12500.031.13844918
CACGAGA12600.030.8913221
CCCACGA12600.030.8913219
AGACGAG12500.030.77846725
ATCTCGT12650.030.76921837
TCTCGTA12650.030.76921838
CTCGTAT12600.030.71275739
CCACGAG12750.030.70435320
ACGAGAG12600.030.53419327
GAGTTGA12650.030.41350431
Produced by FastQC (version 0.11.2)