FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_20.351000000451c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_20.351000000451c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340523
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC154194.528034817031449RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT50631.48683055182763No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT9740.2860306058621591No Hit
GGTGAAGACGGTGAGAAGAAGAAGAAGGAGAAAAAGAAGAAGATTGTTGAA7660.22494809454867956No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG7520.22083677167181073No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT7020.2061534756829935No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC6440.18912085233596557No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC5950.1747312222669247No Hit
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC5470.1606352581176602No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT5380.1579922648396731No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4750.13949131189376343No Hit
CTTGAAAACCATCTTAAAGCAACTCAAAGCTCAAATCGAAATTTCTAGTTT4730.13890398005421073No Hit
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC4480.13156233205980214No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4290.1259826795840516No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4190.12304602038628815No Hit
GTGTTCTTGTACTCTTCTGCCATACTTAATCTTGATTCGATTGAACACTTA4010.11776003383031396No Hit
TCTTAAAGCAACTCAAAGCTCAAATCGAAATTTCTAGTTTCTCTTTATCAT3860.11335504503366879No Hit
TGTGAAAACAGTGGAAGAAGAGAATCAAGGAGTAATGGACAGGATTAAGGC3700.1086563903172473No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3450.10131474232283869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTCG207.028207E-444.997527
TGCCGTC16300.042.92706745
GCCGTCT702.1827873E-1135.3551845
TATTTAG352.8098776E-432.155242
ATGCCGT21800.032.09684444
GTATGCC22650.030.79299442
TATGCCG22750.030.75653543
CGTATGC22950.030.68457441
CTCGTAT22900.030.55507339
TCTCGTA23000.030.42222638
TCGTATG23150.030.32229240
ATCTCGT23150.030.22510737
CGAGACG23650.030.1568923
AGACGAG23700.030.09326725
GATCTCG23150.030.03073336
CGAATAC300.00513255430.0115573
GCCACTC300.005143626629.99833530
CGAGAGT23900.029.93557728
GACGAGA23850.029.90400126
ACGAGAG23850.029.90400127
Produced by FastQC (version 0.11.2)