Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_2.35100000044f7a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25788 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 13368 | 51.83806421591438 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 1471 | 5.704203505506436 | RNA PCR Primer, Index 44 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 526 | 2.0397083914999223 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 154 | 0.5971769815418024 | RNA PCR Primer, Index 44 (95% over 23bp) |
TCTTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 33 | 0.12796649604467195 | No Hit |
CTTATACACATCTCCGAGCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 27 | 0.10469986040018613 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATTCGTATGCCGTCT | 26 | 0.10082208779277184 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1400 | 0.0 | 42.910713 | 45 |
GCCGTCT | 65 | 0.0 | 41.53846 | 45 |
ATGCCGT | 1555 | 0.0 | 38.633442 | 44 |
CTCCGAG | 1620 | 0.0 | 37.916668 | 12 |
TCCGAGC | 1615 | 0.0 | 37.894737 | 13 |
CCACGAG | 1610 | 0.0 | 37.73292 | 20 |
CACGAGA | 1605 | 0.0 | 37.71028 | 21 |
ACGAGAC | 1605 | 0.0 | 37.71028 | 22 |
TCTCCGA | 1630 | 0.0 | 37.684048 | 11 |
CGAGCCC | 1625 | 0.0 | 37.661537 | 15 |
CCGAGCC | 1625 | 0.0 | 37.661537 | 14 |
GAGCCCA | 1625 | 0.0 | 37.523075 | 16 |
ATCTCCG | 1645 | 0.0 | 37.477203 | 10 |
GCCCACG | 1615 | 0.0 | 37.47678 | 18 |
GAGACCC | 1610 | 0.0 | 37.45342 | 24 |
AGCCCAC | 1625 | 0.0 | 37.384613 | 17 |
CCCACGA | 1620 | 0.0 | 37.36111 | 19 |
CGAGACC | 1620 | 0.0 | 37.36111 | 23 |
AGACCCT | 1615 | 0.0 | 37.33746 | 25 |
GACCCTA | 1620 | 0.0 | 37.22222 | 26 |