FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_2.35100000044f7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_2.35100000044f7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25788
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC1336851.83806421591438No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT14715.704203505506436RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG5262.0397083914999223No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT1540.5971769815418024RNA PCR Primer, Index 44 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG330.12796649604467195No Hit
CTTATACACATCTCCGAGCCACGAGACCCTAGAATATCTCGTATGCCGTCT270.10469986040018613RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATTCGTATGCCGTCT260.10082208779277184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14000.042.91071345
GCCGTCT650.041.5384645
ATGCCGT15550.038.63344244
CTCCGAG16200.037.91666812
TCCGAGC16150.037.89473713
CCACGAG16100.037.7329220
CACGAGA16050.037.7102821
ACGAGAC16050.037.7102822
TCTCCGA16300.037.68404811
CGAGCCC16250.037.66153715
CCGAGCC16250.037.66153714
GAGCCCA16250.037.52307516
ATCTCCG16450.037.47720310
GCCCACG16150.037.4767818
GAGACCC16100.037.4534224
AGCCCAC16250.037.38461317
CCCACGA16200.037.3611119
CGAGACC16200.037.3611123
AGACCCT16150.037.3374625
GACCCTA16200.037.2222226