FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_18.35100000045182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_18.35100000045182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263586
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC40781.5471231400757248RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT5230.19841721487484162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT4750.037.89457744
ACCAATA250.002104213235.9998478
CGTATGC5150.034.9513141
TATGCCG5150.034.9513143
GTATGCC5150.034.9513142
CGAGACG5150.034.9513123
CGAGAGT5250.034.7141428
CTCGTAT5200.034.61524239
TCTCGTA5200.034.61524238
GCCCACG5300.033.9621218
AGAGTTG5300.033.9621230
AGACGAG5300.033.9621225
TCGTATG5300.033.9621240
CACGAGA5350.033.6447221
CCCACGA5350.033.6447219
GAGTTGA5350.033.6447231
GATCTCG5450.033.02738636
ACGAGAG5500.032.72713527
ACGAGAC5550.032.43229322
TGCCGTC5550.032.43229345
Produced by FastQC (version 0.11.2)