FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_17.35100000045168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_17.35100000045168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64651
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC1102917.059287559357163RNA PCR Primer, Index 23 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGT13992.1639263120446706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCG3680.5692100663562822No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT3070.4748573107917898No Hit
ATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTTC1330.20571994246028677RNA PCR Primer, Index 23 (95% over 24bp)
CTACTAAAGATACATAACAAGGACACTTTCTGTGTTGGTTAAAGTTTGCAC1290.19953287652163154No Hit
GTACAGTGTGATGTTTTGATATACACTACTTCTGTGGATATACACCATCTT900.13920898361974293No Hit
TTCTAATTCTGTGAAGAATCATGGTGGTATTTTGATGGAGATTGCATGAAT860.13302191768108768No Hit
GAAGGATTATGTCAAGTGACATATGATATGTGGAATAAAGGCTGGGACGAA760.11755425283444959No Hit
TATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCTT740.11446071986512196RNA PCR Primer, Index 23 (95% over 23bp)
AATCAATGGTGAACATTATTTACCACGTTTTCACGGTGATGAAATTGTTAA700.10827365392646672No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTCA206.985721E-444.9976811
ACAGCTC253.851138E-544.9976830
GTTCACT253.851138E-544.9976822
TTGCATA206.985721E-444.9976842
GGTTCAC253.851138E-544.9976821
GTTATAA253.851138E-544.997689
TGCCGTC11950.041.2321845
CAGCTCC356.1594874E-638.5694431
AGTGGTT301.1283913E-437.49806618
AACTGTA301.1283913E-437.49806639
ACTGTAA301.1283913E-437.49806640
GTGGTTC301.1283913E-437.49806619
TAATGCT301.1283913E-437.49806644
ATGCCGT13250.037.18676444
TCCGAGC13650.036.42669313
CCGAGCC13750.036.1617714
AGCCCAC13700.036.12952417
CCCACGA13700.036.12952419
AGACGAG13700.036.12952425
GAGACGA13700.036.12952424
Produced by FastQC (version 0.11.2)