FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_15.35100000045118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_15.35100000045118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270014
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC6052422.415134030087327RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT2936010.873510262430838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG26400.9777270808180317No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT13140.48664143340715665No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5090.1885087439910523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA4310.1596213529668832No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC4000.14814046679061085No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC3640.13480782477945588No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC3560.13184501544364366No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3360.12443799210411313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG3320.12295658743620701No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3190.11814202226551217No Hit
TCTATATGCTCTTCTTCTGTCTCTTTTGTTGTCATGTATGTTTGTTCACTT2870.10629078492226328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC66350.043.50576445
GCCGTCT2100.039.640945
ATGTAAG352.8094018E-432.153181
TAGGGTC651.3907993E-831.1638495
TATATGC1600.030.9474333
TCTATAT1550.030.4936621
ACCCGCT300.005132301230.0096346
GACAGTG300.00514160929.998527
CCCGCTG300.00514160929.998527
ATGCCGT96700.029.8744344
TATGCCG99900.028.9174943
CGTATGC100350.028.89991841
GTATGCC100150.028.84530842
TCGTATG100500.028.83439640
CTCGTAT100350.028.81023639
ATCTCGT100550.028.77530737
TCTCGTA100550.028.77530738
ATTAATC100450.028.75915733
AATCTCG100550.028.7529336
GCTCTTC1800.028.7485798
Produced by FastQC (version 0.11.2)