FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_14.3510000004510b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_14.3510000004510b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381712
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC88972.3308148551787733RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT19720.5166198599991617No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5670.14854130863059062No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT5620.14723142054742844No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4860.12732112168336338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10950.042.94395445
GCCGTCT356.238575E-638.57030545
ATGCCGT12900.036.45242744
GACGTTC13600.035.56881726
CGAGACG13550.035.5340223
TATGCCG13400.035.42807443
CGTATGC13550.035.20192741
AGACGTT13850.034.9267825
GTATGCC13600.034.9070742
CGTTCAT13900.034.80114728
CACGAGA14000.034.71327621
ACGAGAC14000.034.55256722
AATCTCG13900.034.47741336
CTCGTAT13850.034.4394339
TCTCGTA13950.034.3538438
ACGTTCA14200.034.0659127
TCGTATG14100.033.98837340
GTTCATT14250.033.9463829
ATCTCGT14200.033.74901637
GAGACGT14300.033.6703524
Produced by FastQC (version 0.11.2)