FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_13.351000000450e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_13.351000000450e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400743
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC123403.079280236959847RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT57851.4435685713786641No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG8280.20661621038920208No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT5950.14847420915649182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10750.042.065845
GTCCGGG250.002105159236.001131
GGGAACG250.00210644935.99660513
GGAACGC250.00210644935.99660514
ACGCCAT554.159094E-628.63366317
AACGCCA406.162477E-428.12234716
ATGCCGT17350.025.93415544
TACCTTA450.001224051125.0038836
TTTCGTG450.001224942325.00076338
GTATAGA551.3599727E-424.561531
ATTAATC18350.024.52390933
TATGCCG18350.024.5208543
GTATGCC18400.024.45421642
CACGCTA651.5216459E-524.22848313
CGTATGC18600.024.19126741
CTCGTAT18600.024.19126739
CGAGACG19000.024.15561723
GACGTTC18650.024.12942526
AGACGTT18850.024.11214325
CGTTCAT18900.024.04835128
Produced by FastQC (version 0.11.2)