FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_12.351000000450c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_12.351000000450c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423553
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC134073.1653653734007317RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT37110.8761595361147247No Hit
CCTTAATCCTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACAT9310.21980720240442164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG7810.18439250813947725No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA6840.16149100584814652No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT6270.14803342202746764No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA4970.11734068699784915No Hit
GTTTTCACATGATCTCCTTCAACAATCTTCTTCTTTTTCTCCTTCTTCTTC4920.11616019718901766No Hit
GTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTCACATGATCTCCTTC4700.11096604203015914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAA207.028419E-444.9997618
TGCCGTC15750.041.85692245
ACAGCGC250.00210566435.99981337
ATGCCGT20000.032.9623344
TATGCCG20900.031.54289643
CGTATGC21050.031.53190241
GACGTTC21000.031.3926926
AGACGTT21100.031.35054425
ACGTTCA21050.031.31812727
CGAGACG21150.031.27643223
GTATGCC21100.031.2439142
CTCGTAT21250.031.2351339
ACGAGAC21400.031.1213322
ATCTCGT21200.031.09653537
TCGTATG21350.030.98344440
CGTTCAT21400.030.8059128
CACGAGA21600.030.72900621
CCCACGA21600.030.72900619
TCTCGTA21700.030.69108438
AATCTCG21400.030.5956336
Produced by FastQC (version 0.11.2)