FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_11.351000000450ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_11.351000000450ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357026
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC103022.885504136953611RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT16550.46355167410776804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.1181986746063312No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4160.11651812473041179No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA4070.11399729991653268No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG3860.10811537535081478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10750.041.23076245
ATGCCGT12450.035.60085744
TATGCCG12750.034.7631943
GTATGCC12850.034.6677542
CGTATGC12950.034.40004341
CTCGTAT12900.034.35896739
ATTAATC13000.034.2677433
GACGTTC12950.034.0525726
CATTAAT13250.033.7909832
GTATTAA401.5535536E-533.762721
CGAGACG13200.033.74852823
CGTTCAT13150.033.70575328
ACGTTCA13100.033.66265527
TCTCGTA13200.033.57808338
GTTCATT13200.033.57808329
TCATTAA13350.033.53786531
TTCATTA13150.033.5346630
AGACGTT13200.033.40763525
TCGTATG13400.033.2448240
AATCTCG13350.033.200836