Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_11.351000000450ad.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 357026 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC | 10302 | 2.885504136953611 | RNA PCR Primer, Index 45 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT | 1655 | 0.46355167410776804 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 422 | 0.1181986746063312 | No Hit |
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG | 416 | 0.11651812473041179 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA | 407 | 0.11399729991653268 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG | 386 | 0.10811537535081478 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1075 | 0.0 | 41.230762 | 45 |
ATGCCGT | 1245 | 0.0 | 35.600857 | 44 |
TATGCCG | 1275 | 0.0 | 34.76319 | 43 |
GTATGCC | 1285 | 0.0 | 34.66775 | 42 |
CGTATGC | 1295 | 0.0 | 34.400043 | 41 |
CTCGTAT | 1290 | 0.0 | 34.358967 | 39 |
ATTAATC | 1300 | 0.0 | 34.26774 | 33 |
GACGTTC | 1295 | 0.0 | 34.05257 | 26 |
CATTAAT | 1325 | 0.0 | 33.79098 | 32 |
GTATTAA | 40 | 1.5535536E-5 | 33.76272 | 1 |
CGAGACG | 1320 | 0.0 | 33.748528 | 23 |
CGTTCAT | 1315 | 0.0 | 33.705753 | 28 |
ACGTTCA | 1310 | 0.0 | 33.662655 | 27 |
TCTCGTA | 1320 | 0.0 | 33.578083 | 38 |
GTTCATT | 1320 | 0.0 | 33.578083 | 29 |
TCATTAA | 1335 | 0.0 | 33.537865 | 31 |
TTCATTA | 1315 | 0.0 | 33.53466 | 30 |
AGACGTT | 1320 | 0.0 | 33.407635 | 25 |
TCGTATG | 1340 | 0.0 | 33.24482 | 40 |
AATCTCG | 1335 | 0.0 | 33.2008 | 36 |