FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_10.35100000045083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_10.35100000045083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442636
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC66521.5028149540480213RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT10840.2448964837925519No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG4950.11183003641818559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9350.036.33598343
ATGCCGT9400.036.14270844
CGTATGC9550.036.04621541
AGGTAGT250.002105971635.99909642
TGCCGTC9450.035.95147745
GACGTTC9700.035.72075326
TCGTATG9650.035.67268440
GTATGCC9550.035.5750242
ACGTTCA9750.035.53756727
GCCCACG9850.035.1767818
AGACGTT9850.035.1767825
CGAGACG9850.035.1767823
ATTAATC9900.034.99912333
CACGAGA10000.034.64912821
CTCGTAT10000.034.42413339
CCCACGA10100.034.30607219
CGTTCAT10150.034.13707428
GTTCATT10100.034.08330529
ATCTCGT10150.033.91540537
AATCTCG10200.033.74915336
Produced by FastQC (version 0.11.2)