FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pc_1.35100000044f51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pc_1.35100000044f51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17842
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC540530.29368904831297No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT4412.4716959982064792RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT1430.8014796547472256RNA PCR Primer, Index 44 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1280.717408362291223No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC1270.7118036094608228RNA PCR Primer, Index 37 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT470.2634233830288084RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT230.1289093150992041RNA PCR Primer, Index 44 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6700.041.3059745
CGAGCCC7250.038.79310215
TCCGAGC7250.038.79310213
CTCCGAG7250.038.79310212
AGCCCAC7200.038.7517
GCCCACG7200.038.7518
CCACGAG7200.038.7520
CACGAGA7200.038.7521
CCCACGA7200.038.7519
ACGAGAC7200.038.7522
ACCCTAG7200.038.7527
GACCCTA7200.038.7526
ATGCCGT7150.038.70629544
GAATATC7150.038.70629533
CTAGAAT7150.038.70629530
TAGAATA7100.038.6619731
GAGCCCA7300.038.52739716
CCGAGCC7300.038.52739714
GAGACCC7300.038.52739724
CGTATGC7250.038.48275841