Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pc_1.35100000044f51.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17842 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 5405 | 30.29368904831297 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 441 | 2.4716959982064792 | RNA PCR Primer, Index 44 (95% over 21bp) |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 143 | 0.8014796547472256 | RNA PCR Primer, Index 44 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 128 | 0.717408362291223 | No Hit |
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC | 127 | 0.7118036094608228 | RNA PCR Primer, Index 37 (95% over 23bp) |
TATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTT | 47 | 0.2634233830288084 | RNA PCR Primer, Index 37 (95% over 22bp) |
CTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 23 | 0.1289093150992041 | RNA PCR Primer, Index 44 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 670 | 0.0 | 41.30597 | 45 |
CGAGCCC | 725 | 0.0 | 38.793102 | 15 |
TCCGAGC | 725 | 0.0 | 38.793102 | 13 |
CTCCGAG | 725 | 0.0 | 38.793102 | 12 |
AGCCCAC | 720 | 0.0 | 38.75 | 17 |
GCCCACG | 720 | 0.0 | 38.75 | 18 |
CCACGAG | 720 | 0.0 | 38.75 | 20 |
CACGAGA | 720 | 0.0 | 38.75 | 21 |
CCCACGA | 720 | 0.0 | 38.75 | 19 |
ACGAGAC | 720 | 0.0 | 38.75 | 22 |
ACCCTAG | 720 | 0.0 | 38.75 | 27 |
GACCCTA | 720 | 0.0 | 38.75 | 26 |
ATGCCGT | 715 | 0.0 | 38.706295 | 44 |
GAATATC | 715 | 0.0 | 38.706295 | 33 |
CTAGAAT | 715 | 0.0 | 38.706295 | 30 |
TAGAATA | 710 | 0.0 | 38.66197 | 31 |
GAGCCCA | 730 | 0.0 | 38.527397 | 16 |
CCGAGCC | 730 | 0.0 | 38.527397 | 14 |
GAGACCC | 730 | 0.0 | 38.527397 | 24 |
CGTATGC | 725 | 0.0 | 38.482758 | 41 |