Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_96.35100000044f37.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 35021 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 9133 | 26.078638531167016 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 630 | 1.7989206476114332 | RNA PCR Primer, Index 41 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC | 400 | 1.142171839753291 | RNA PCR Primer, Index 34 (95% over 23bp) |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 323 | 0.9223037606007823 | RNA PCR Primer, Index 41 (95% over 23bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 152 | 0.43402529910625054 | No Hit |
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT | 112 | 0.31980811513092144 | RNA PCR Primer, Index 34 (95% over 22bp) |
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 52 | 0.1484823391679278 | RNA PCR Primer, Index 41 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGTCTT | 30 | 2.105582E-6 | 45.000004 | 44 |
TGCCGTC | 980 | 0.0 | 42.015305 | 45 |
ATGCCGT | 1045 | 0.0 | 39.401913 | 44 |
CGTATGC | 1095 | 0.0 | 38.013702 | 41 |
ACTCGAC | 1105 | 0.0 | 37.873302 | 27 |
CACGAGA | 1105 | 0.0 | 37.873302 | 21 |
AGACTCG | 1105 | 0.0 | 37.873302 | 25 |
GACTCGA | 1105 | 0.0 | 37.873302 | 26 |
AGCCCAC | 1100 | 0.0 | 37.840908 | 17 |
CCCACGA | 1100 | 0.0 | 37.840908 | 19 |
CGAGCCC | 1100 | 0.0 | 37.840908 | 15 |
TCGTATG | 1100 | 0.0 | 37.840908 | 40 |
GTATGCC | 1095 | 0.0 | 37.808224 | 42 |
TCTCGTA | 1095 | 0.0 | 37.808224 | 38 |
CTCGTAT | 1095 | 0.0 | 37.808224 | 39 |
TATGCCG | 1090 | 0.0 | 37.77523 | 43 |
ACGAGAC | 1110 | 0.0 | 37.7027 | 22 |
CGAGACT | 1110 | 0.0 | 37.7027 | 23 |
TCCGAGC | 1110 | 0.0 | 37.7027 | 13 |
GAGACTC | 1110 | 0.0 | 37.7027 | 24 |