FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_96.35100000044f37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_96.35100000044f37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35021
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC913326.078638531167016No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT6301.7989206476114332RNA PCR Primer, Index 41 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC4001.142171839753291RNA PCR Primer, Index 34 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT3230.9223037606007823RNA PCR Primer, Index 41 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG1520.43402529910625054No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT1120.31980811513092144RNA PCR Primer, Index 34 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT520.1484823391679278RNA PCR Primer, Index 41 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT302.105582E-645.00000444
TGCCGTC9800.042.01530545
ATGCCGT10450.039.40191344
CGTATGC10950.038.01370241
ACTCGAC11050.037.87330227
CACGAGA11050.037.87330221
AGACTCG11050.037.87330225
GACTCGA11050.037.87330226
AGCCCAC11000.037.84090817
CCCACGA11000.037.84090819
CGAGCCC11000.037.84090815
TCGTATG11000.037.84090840
GTATGCC10950.037.80822442
TCTCGTA10950.037.80822438
CTCGTAT10950.037.80822439
TATGCCG10900.037.7752343
ACGAGAC11100.037.702722
CGAGACT11100.037.702723
TCCGAGC11100.037.702713
GAGACTC11100.037.702724