Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_95.35100000044f1d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11861 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 3453 | 29.112216507882977 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 229 | 1.9306972430655087 | RNA PCR Primer, Index 41 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 203 | 1.7114914425427872 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 24 | 0.20234381586712755 | RNA PCR Primer, Index 41 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 365 | 0.0 | 41.301373 | 45 |
ATGCCGT | 385 | 0.0 | 39.155846 | 44 |
CACGAGA | 405 | 0.0 | 38.88889 | 21 |
CCACGAG | 400 | 0.0 | 38.8125 | 20 |
CCCACGA | 400 | 0.0 | 38.8125 | 19 |
AGCCCAC | 395 | 0.0 | 38.734177 | 17 |
GCCCACG | 395 | 0.0 | 38.734177 | 18 |
ACGAGAC | 395 | 0.0 | 38.734177 | 22 |
CGAGACT | 395 | 0.0 | 38.734177 | 23 |
CTCGACG | 405 | 0.0 | 38.333336 | 28 |
GAGCCCA | 405 | 0.0 | 38.333336 | 16 |
CGACGGC | 405 | 0.0 | 38.333336 | 30 |
ACTCGAC | 400 | 0.0 | 38.25 | 27 |
GACGGCA | 400 | 0.0 | 38.25 | 31 |
GACTCGA | 400 | 0.0 | 38.25 | 26 |
GAGACTC | 400 | 0.0 | 38.25 | 24 |
GGCATCT | 390 | 0.0 | 38.076923 | 34 |
CATCTCG | 390 | 0.0 | 38.076923 | 36 |
CGGCATC | 390 | 0.0 | 38.076923 | 33 |
GCATCTC | 390 | 0.0 | 38.076923 | 35 |