FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_95.35100000044f1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_95.35100000044f1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11861
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC345329.112216507882977No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT2291.9306972430655087RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG2031.7114914425427872No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT240.20234381586712755RNA PCR Primer, Index 41 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3650.041.30137345
ATGCCGT3850.039.15584644
CACGAGA4050.038.8888921
CCACGAG4000.038.812520
CCCACGA4000.038.812519
AGCCCAC3950.038.73417717
GCCCACG3950.038.73417718
ACGAGAC3950.038.73417722
CGAGACT3950.038.73417723
CTCGACG4050.038.33333628
GAGCCCA4050.038.33333616
CGACGGC4050.038.33333630
ACTCGAC4000.038.2527
GACGGCA4000.038.2531
GACTCGA4000.038.2526
GAGACTC4000.038.2524
GGCATCT3900.038.07692334
CATCTCG3900.038.07692336
CGGCATC3900.038.07692333
GCATCTC3900.038.07692335