FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_94.35100000044ef2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_94.35100000044ef2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC68727.326968973747018No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT351.3922036595067622RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG341.352426412092283No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT70.27844073190135243RNA PCR Primer, Index 41 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC40.15910898965791567No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCGGCATCTCGTATGCCGTC40.15910898965791567No Hit
CTTATACACATCTCCGAGCCACGAGACTCGACGGCATCTCGTATGCCGTCT30.11933174224343676RNA PCR Primer, Index 41 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT30.11933174224343676RNA PCR Primer, Index 41 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG600.045.00000428
ACTCGAC600.045.00000427
GGCATCT700.045.00000434
TCGACGG600.045.00000429
GACGGCA650.045.00000431
CGACGGC650.045.00000430
CGAGACT700.045.00000423
AGACTCG700.045.00000425
CGGCATC700.045.00000433
GCATCTC700.045.00000435
ACGGCAT650.045.00000432
GACTCGA600.045.00000426
GAGACTC700.045.00000424
AGCCCAC750.045.017
GTATGCC750.045.042
ATCTCGT750.045.037
ATCTCCG750.045.010
CACATCT750.045.07
GCCCACG750.045.018
TGCCGTC750.045.045