Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_94.35100000044ef2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2514 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC | 687 | 27.326968973747018 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 35 | 1.3922036595067622 | RNA PCR Primer, Index 41 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG | 34 | 1.352426412092283 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 7 | 0.27844073190135243 | RNA PCR Primer, Index 41 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC | 4 | 0.15910898965791567 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTCGCCGGCATCTCGTATGCCGTC | 4 | 0.15910898965791567 | No Hit |
CTTATACACATCTCCGAGCCACGAGACTCGACGGCATCTCGTATGCCGTCT | 3 | 0.11933174224343676 | RNA PCR Primer, Index 41 (95% over 23bp) |
CTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT | 3 | 0.11933174224343676 | RNA PCR Primer, Index 41 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGACG | 60 | 0.0 | 45.000004 | 28 |
ACTCGAC | 60 | 0.0 | 45.000004 | 27 |
GGCATCT | 70 | 0.0 | 45.000004 | 34 |
TCGACGG | 60 | 0.0 | 45.000004 | 29 |
GACGGCA | 65 | 0.0 | 45.000004 | 31 |
CGACGGC | 65 | 0.0 | 45.000004 | 30 |
CGAGACT | 70 | 0.0 | 45.000004 | 23 |
AGACTCG | 70 | 0.0 | 45.000004 | 25 |
CGGCATC | 70 | 0.0 | 45.000004 | 33 |
GCATCTC | 70 | 0.0 | 45.000004 | 35 |
ACGGCAT | 65 | 0.0 | 45.000004 | 32 |
GACTCGA | 60 | 0.0 | 45.000004 | 26 |
GAGACTC | 70 | 0.0 | 45.000004 | 24 |
AGCCCAC | 75 | 0.0 | 45.0 | 17 |
GTATGCC | 75 | 0.0 | 45.0 | 42 |
ATCTCGT | 75 | 0.0 | 45.0 | 37 |
ATCTCCG | 75 | 0.0 | 45.0 | 10 |
CACATCT | 75 | 0.0 | 45.0 | 7 |
GCCCACG | 75 | 0.0 | 45.0 | 18 |
TGCCGTC | 75 | 0.0 | 45.0 | 45 |