FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_93.35100000044ee5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_93.35100000044ee5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28577
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC24688.636315918395914No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT2790.9763096196241734RNA PCR Primer, Index 41 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT750.2624488224796165RNA PCR Primer, Index 41 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG580.2029604227175701No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC540.18896315218532386RNA PCR Primer, Index 34 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3000.039.00000445
CGAGCCC3500.034.07142615
CCGAGCC3500.034.07142614
TCCGAGC3500.034.07142613
GCCCACG3450.033.91304418
GAGCCCA3450.033.91304416
TCTCCGA3550.033.5915511
CTCCGAG3550.033.5915512
CTCGACG3500.033.4285728
AGCCCAC3500.033.4285717
GGCATCT3500.033.4285734
TCGACGG3500.033.4285729
CCACGAG3500.033.4285720
CATCTCG3500.033.4285736
GACGGCA3500.033.4285731
CACGAGA3500.033.4285721
CCCACGA3500.033.4285719
ACGAGAC3500.033.4285722
CGAGACT3500.033.4285723
CGGCATC3500.033.4285733
Produced by FastQC (version 0.11.2)