FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_92.35100000044ecb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_92.35100000044ecb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGC23.508771929824561No Hit
CTTCACTAGTAGATATTCTCCAAGTTGCTTCCACTCAGCTGTCGATCTGTC11.7543859649122806No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAGAG11.7543859649122806RNA PCR Primer, Index 36 (96% over 29bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAAGCCACCTC11.7543859649122806RNA PCR Primer, Index 36 (96% over 29bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAACACCCGA11.7543859649122806Illumina PCR Primer Index 8 (96% over 27bp)
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGTCTCT11.7543859649122806No Hit
GTACTTTGATGTTTCAGTTCCCCCGGTTCGCCCCATTAACCTATGGCGTCA11.7543859649122806No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGAGAAATCGG11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGCTGTCTCTTATAC11.7543859649122806No Hit
GCTATATGCTTAACACATGCAAGTCGAACGTTGTTCTCGGGGAGCTAGGCA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGCTGTTATACATCTCGTATGCCGTCT11.7543859649122806RNA PCR Primer, Index 39 (100% over 21bp)
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT11.7543859649122806No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAACAAATTATAAAAAAAA11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTACACGTCAT11.7543859649122806Illumina PCR Primer Index 5 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCACACAGCGC11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
AATTTGGACATTGTGGGGGTGGGGCTGGTGAGAAAATGAGGGGTATGGAAA11.7543859649122806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTT11.7543859649122806No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGCCCTCTCTC11.7543859649122806Illumina PCR Primer Index 8 (96% over 27bp)
CCTTTTGTCAGTTCTTGATGATGATCCACCAAAACAAGGATGCAGCGAGTC11.7543859649122806No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTAACCAACCC11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAGAAACGGACA11.7543859649122806RNA PCR Primer, Index 36 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGACACTCTACGGCATCGCGTATGCTCTC11.7543859649122806No Hit
CCTTTGTGCAATGACGTCACAATAGGAGATTGCTAATGCTCTGTCTCTTAT11.7543859649122806No Hit
GCAGAGTACGGGGTATCAACGCAGAGTACGGGAGTGGCTGTCTCTTATACA11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCGAGACAGGATGTGATCTCGTATGCCGTCTT11.7543859649122806TruSeq Adapter, Index 5 (95% over 21bp)
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC11.7543859649122806No Hit
GAGATGCTTAGCCATGATAACCTCATCTGTAGGATTCACTACACCATCTTC11.7543859649122806No Hit
TCCCTGCGCTACGGCGTGGTCAAGATGAACGTTGACACGGACACCCAGTAC11.7543859649122806No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTTTGCATT11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTC11.7543859649122806No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC11.7543859649122806No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACCACACC11.7543859649122806Illumina PCR Primer Index 5 (96% over 28bp)
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATT11.7543859649122806No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT11.7543859649122806No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTG11.7543859649122806No Hit
CCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTCT11.7543859649122806No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACAA11.7543859649122806Illumina PCR Primer Index 5 (96% over 28bp)
CGCCGAACATGGATTTCTGTCCGTTGGCCAGCGCATAGGAATTGCCCAACG11.7543859649122806No Hit
GCCTGGACTAACACCATTGCAGAATCATCTGCCTGTCTCTTATACACATCT11.7543859649122806No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT11.7543859649122806No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAT11.7543859649122806No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGTACCCC11.7543859649122806No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAACTCTAGA11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
ATATNAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACTGTTTCT11.7543859649122806Illumina PCR Primer Index 8 (96% over 27bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATATAACA11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
GTACGGGAAGCAGTGGTATCAAGCAGAAAAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC11.7543859649122806No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGCTTTCT11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCATC11.7543859649122806RNA PCR Primer, Index 27 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC11.7543859649122806No Hit
GCATCTTAGTCGTTGGATCTCAGCAGGTAAGTGGTCGAATGATAGCCGGTG11.7543859649122806No Hit
CAACCACACATTTGGGAAGATGTCCCTGGAATTAAAGGGGCGTTTATAGTC11.7543859649122806No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATCCAGT11.7543859649122806No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAATCA11.7543859649122806Illumina PCR Primer Index 8 (96% over 27bp)
GGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAA11.7543859649122806No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAGAAG11.7543859649122806Illumina PCR Primer Index 8 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.018
ACTGTAT50.045.033
GTATGCC50.045.043
ATCTCGT50.045.038
ATCTCCG50.045.011
CACATCT50.045.08
GCCCACG50.045.019
TATACAC50.045.04
AACTGTA50.045.032
CAACTGT50.045.031
CCACGAG50.045.021
TATGCCG50.045.044
CATCTCC50.045.010
GTATCTC50.045.036
GAGCCCA50.045.017
TCAACTG50.045.030
CACGAGA50.045.022
CCCACGA50.045.020
ACATCTC50.045.09
TTATACA50.045.03
Produced by FastQC (version 0.11.2)