FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_91.35100000044ea2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_91.35100000044ea2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC34.166666666666666No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA22.7777777777777777RNA PCR Primer, Index 27 (96% over 28bp)
GTGAATCACTTGAGCCCTGATACATTTTCGGAGCCATGGAGCTAGACCAGT11.3888888888888888No Hit
GTTCTGAGCTGACTGTTCGACGCCCCGGGGAAAGCTCCCGAGAGAGCCGTT11.3888888888888888No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGATAGCATCTCGTATGCCGTC11.3888888888888888No Hit
ATGTATGGGAATCAAAGGCTAACGGTACATTTGCTGTTTCCGAGGATACAT11.3888888888888888No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAAAAAAAAA11.3888888888888888RNA PCR Primer, Index 27 (96% over 28bp)
GGTTATGCGTACTTGGTGCAAACTTGCCGTGCTCTCTCTTATACACATCTC11.3888888888888888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAACAA11.3888888888888888No Hit
CTAGAAACAGTGCATGAGATTCTCGAGAGATTAGCGGCTCATTTGAAGGCA11.3888888888888888No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.3888888888888888No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCT11.3888888888888888No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAAAACAC11.3888888888888888Illumina PCR Primer Index 8 (96% over 27bp)
CCACTGCCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA11.3888888888888888No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCCCC11.3888888888888888No Hit
GGCAGCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTGA11.3888888888888888No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA11.3888888888888888No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC11.3888888888888888No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCGGACACCC11.3888888888888888RNA PCR Primer, Index 27 (96% over 28bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCCGC11.3888888888888888No Hit
GTCTTTAAGATCCTCACCGTCCTTGATGTGGGGATATCGACCCTGAACGAG11.3888888888888888No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACGGCATCTCGTATGCCGTC11.3888888888888888No Hit
GTACGGGCGAGGTTAAGCGACTAAGCGAACACGGTGGATGCACTGGCAGTC11.3888888888888888No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGTCTGGGCA11.3888888888888888RNA PCR Primer, Index 27 (96% over 28bp)
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATCGGTTAATGA11.3888888888888888No Hit
CTTTTGTTCCCCATACTGCTGGACGATACTCTGTGAAGAGGTTCAGAAACT11.3888888888888888No Hit
CGTCTGCAGATGTCATAGATAGCTTCATTGTCGCGGAGGATAGAGACATCA11.3888888888888888No Hit
AAAGATAACTCTTCCCGAGGCCCACGCCGACGTCTCCGGACTCCCTAACGT11.3888888888888888No Hit
GTTCAAGCCAAGCTTCACGTGCACAATTCTGGCGGCTTTGCAGGAGATTAC11.3888888888888888No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCCAGCACAT11.3888888888888888RNA PCR Primer, Index 27 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTACACCAC11.3888888888888888No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAAC11.3888888888888888No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGACT11.3888888888888888No Hit
TCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG11.3888888888888888No Hit
TAAGAATACCGTCACAGATTTTCGATAACTCAGATTCGAACTTGCTTCTGT11.3888888888888888No Hit
GATAACCATTTCTCTCCCCGAAATCGAGACTCCTGAATTTTGGCCGGACCT11.3888888888888888No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC11.3888888888888888No Hit
ATCAACGCAGAGTACGGGAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAA11.3888888888888888No Hit
CACCATTACCTTCGAAGACTTCATCACTGGTGCTTTCTGATCGAAAGAGCA11.3888888888888888No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC11.3888888888888888No Hit
TGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACCTCTCCGAG11.3888888888888888No Hit
GACCGACGCGGATTACGGTGGCGGCCTACGCCCGGGCTTTTGATACGCTTG11.3888888888888888No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTATAATTCTT11.3888888888888888RNA PCR Primer, Index 27 (96% over 28bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA11.3888888888888888No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATAC11.3888888888888888No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATCTGTATCTCGTATGCCGTC11.3888888888888888No Hit
CAGCTGTATGCCGCCATTGGTGTTTTGTTGTTTACAACCATCTTGCTCTTC11.3888888888888888No Hit
ATCTAGAACAAGCTGGTTGGAGCCACGAACTAGAACAGATGTAGTTCCTGT11.3888888888888888No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTCCCCCCCGGTAT11.3888888888888888No Hit
GCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCT11.3888888888888888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA11.3888888888888888No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAC11.3888888888888888No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.3888888888888888No Hit
ATATCCGATCTTGGTTGGCCTTTCACCCCTAGCCACAAGTCATCCCCGTAT11.3888888888888888No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAGCACGTC11.3888888888888888Illumina PCR Primer Index 8 (96% over 27bp)
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATAC11.3888888888888888No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGACTGTCTCT11.3888888888888888No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGCATGCCGTC11.3888888888888888No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC11.3888888888888888No Hit
AAAAAAAAAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATACTGTCTCTT11.3888888888888888No Hit
TGATGACAGAGCTCTTGAGACTGCCTTCGCTCAATACGGCGACTGTCTCTT11.3888888888888888No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA11.3888888888888888Illumina PCR Primer Index 8 (96% over 27bp)
GACTTACACCAAGCCGAAGAAGATCAAGCACAAGCATAAGAAGGTTAAGCC11.3888888888888888No Hit
AAAAAAAAGAGATCCAGAATTAGATGATAAGTTGCAAGAACATTATGAGCA11.3888888888888888No Hit
CGCTTAAAGATGCCGTGCGCAGAGGCCTTCCGGTGCCTGTCTCTTATACAC11.3888888888888888No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC11.3888888888888888No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCCC11.3888888888888888No Hit
CCATCGGAGAGAGAAAACAATCAATAAGCGACCATGTTGGTGTACCAAGAT11.3888888888888888No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATACTCCTC11.3888888888888888RNA PCR Primer, Index 27 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG50.045.022
ACGCAGA50.045.023
TCAACGC50.045.020
CAGAGTA50.045.026
AGAGTAC50.045.027
GCAGAGT50.045.025
CGCAGAG50.045.024
ATCAACG50.045.019
CAACGCA50.045.021
TGGTATC100.022.515
TACGGAA100.022.54
AGTGGTA100.022.513
GAAGCAG100.022.58
GTATCAA100.022.517
AGCAGTG100.022.510
AGTACGG100.022.52
CGGAAGC100.022.56
GCAGTGG100.022.511
GAGTACG100.022.51
GGAAGCA100.022.57
Produced by FastQC (version 0.11.2)