FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_90.35100000044e7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_90.35100000044e7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC44.395604395604396No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC22.197802197802198No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT22.197802197802198No Hit
AAAAAGTACTCTGCGTTGATACCCCCGTACCTGTCTCTTATACACATCTCC11.098901098901099No Hit
GTACTCTGCGTTGATACCACTGCCCCGTACTCTGCGTTGATACCACTGCTT11.098901098901099No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCG11.098901098901099No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA11.098901098901099No Hit
GAAGCAAACATAGGTGGGGTGGAATGGCGGCAAATAAAGGATAATGATTTC11.098901098901099No Hit
GCGAAACAGCAAGCTGTTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCC11.098901098901099No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG11.098901098901099No Hit
GTCTAATTCAGACTGCTGCTCAGTAATTTGTGAACAGTGGAATTTTGATAC11.098901098901099No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTG11.098901098901099No Hit
GTTCAAAGAGGCCTTCAACATGATTGATCAGAACAGAGATGGCTTCATCGA11.098901098901099No Hit
CCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGC11.098901098901099No Hit
GTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCT11.098901098901099No Hit
TCTTGATTCAGTGCTCACTTGGCTTTTATAACCTTTAACTATTGTTAAGAG11.098901098901099No Hit
CGACATCTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAAT11.098901098901099No Hit
TTACAATACCATGTTCGATTGGATACTTCAGAGTGAGGATACCTCTCTTGG11.098901098901099No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACTGCATCTCGTATGCCGTC11.098901098901099No Hit
GGAGGTGACATATCAGACTCCAGCTTCTCCTCGGGCTCTGGTTCTTCTCCA11.098901098901099No Hit
CTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACCCACAAACCAACCAC11.098901098901099Illumina Single End Adapter 1 (100% over 22bp)
GAGATAAGAGAGACAGAGATGAGACAGAGAAAGAGACAGAGAGAAAGACAG11.098901098901099No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTTTATCAAA11.098901098901099RNA PCR Primer, Index 36 (96% over 29bp)
CTGGAAAGCACAAAACCGCAGACATCAACACATTCTCTTGGGGAGTCGCGA11.098901098901099No Hit
AGATTATATTTATTACCTAATCGTTCATAATAATTTTTAACATATGGTTTT11.098901098901099No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAAAAAAAAAAAAAAAATAAAAAGA11.098901098901099No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGACCGCATCCCGTATGCCGTC11.098901098901099No Hit
AATCTCAAGGTGTCGATCAAGCTCGATCGCACCGTAGCTACGAATTTTGAG11.098901098901099No Hit
CTTGCTGCCTTACCTGGCACTTTTATGTTATGGAGACCTGTCCCTTATACC11.098901098901099No Hit
CCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGTGTTG11.098901098901099No Hit
TTCGAGAAGTGTAGTGTAAACATGTTGTTACACAACTGTCTCTTATACACA11.098901098901099No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT11.098901098901099No Hit
GTATCAACGCAGAGTACGGGCTGTCTCTTATACACATCTCCGAGCCCACGA11.098901098901099No Hit
GGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAAC11.098901098901099No Hit
GCCGGGGGACGGACTGGGAACGGCTCTCTCGGGAGCTTTCCCCGGGCTGTT11.098901098901099No Hit
GAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
CTTATACACATCTCCGAGCCCACAGACTGTTATACATCTCGTATGCCGTCT11.098901098901099RNA PCR Primer, Index 39 (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTACAACCCCGTACT11.098901098901099No Hit
GTATTCTGAGCGGCTCCTCTGTTGCTTCCCCAGTGGTCATCTCCGGGAGGC11.098901098901099No Hit
CTTTGACGCTGAGGGTGCTGGGAAGCGGCAAGAGTGGGTCGACCGTTTCGT11.098901098901099No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC11.098901098901099No Hit
AGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTAGGTGAG11.098901098901099No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAGCATCTCGTATGCCGTC11.098901098901099No Hit
TTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTCT11.098901098901099No Hit
ACAGAGGGCTTGGCAGCAGCAGAAGAGAGTCTCGACCTCTTCTTTGCCAAA11.098901098901099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11.098901098901099No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT11.098901098901099No Hit
GAACTAGGGGTCGGCAGAAAGGATTATGGGTGGAAAACATTGGCTCTTCCT11.098901098901099No Hit
TAGATGGTCTGTCCTTTTTGTGATTTCTGTATAGGACCTGTCTCTTATACA11.098901098901099No Hit
CCATCTGACTTCAGCACCGAGAGCAGTTAGGGTTTCGATGAGTACGGCGGT11.098901098901099No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCGTATGCCGTC11.098901098901099RNA PCR Primer, Index 36 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT11.098901098901099No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
CACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAA11.098901098901099No Hit
CCACAATATTCTGCATGTTTTATGTCTGAAGCATGGGGAAAACCTTTACAA11.098901098901099No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACAAAAAAAA11.098901098901099No Hit
ATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGGTCGGATGC11.098901098901099No Hit
CTTATACACATCTCCGAGCCCGCGAGACTCAACTGTATCTCGTATGCCGTC11.098901098901099No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGA11.098901098901099No Hit
GGTATCAACGCTGAGTACGGGCACATGTGGTCAGGTAGAGAATACCAAGGC11.098901098901099No Hit
CAACGAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGATACCCCTCCCCC11.098901098901099No Hit
GCGGAATCTATATAGGAATGTGATGTTAGAGGACTACAGTAAACTGGTCTT11.098901098901099No Hit
GTATTACCATCTTTCCCAAGATATGACGTGAGTATTACTGGAACATCTTGG11.098901098901099No Hit
CTTGGACACTGCTCGCAGTTAGCATTGCCACCGCAATGGCAAGGTACTTGC11.098901098901099No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT11.098901098901099No Hit
CGCCCTAGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGAGGCCTGG11.098901098901099No Hit
ATGGATCTTTTGCATACCAATGCAAACTTGTACCACCACCTCTAATGATGA11.098901098901099No Hit
TATCAACGCAGAGTACGGGAAGGGTATCAACGCAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTCCCCGTACTCTGCTTTGA11.098901098901099No Hit
CTTATACACATCTGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTT11.098901098901099Illumina PCR Primer Index 3 (95% over 21bp)
ATCTACCGTTGGTGTTAAAGGGAGAACTGCGAGGAGCCAACCCTAGTACGA11.098901098901099No Hit
AGTCAGATGTGAAATCCCCGGGCTCAACCAGGGAACTGCATCTGATACTGG11.098901098901099No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTTTTTTTT11.098901098901099No Hit
TGAACAGACACTTCTCAAAAGATGACATACAAGCAGCCAACAAACATATGA11.098901098901099No Hit
GTTTTATGAAGTCTTGGATTGTCGGTAATTGGACTGTCTCTTATACACATC11.098901098901099No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATGATTAGA11.098901098901099Illumina PCR Primer Index 8 (96% over 27bp)
ACCTTTTCCAGCATAAGATTTTTCATAGCTCAGTGTGTATTCTGTCTCTTA11.098901098901099No Hit
AGACGGAACCTTGGCGACGGGTTTACCACCTGGTAAGACATTGTCGGGAAG11.098901098901099No Hit
TGCTACGGGTTATCCAGGGATGAAAATCTTACAGTTTGGCTCTGTCTCTTA11.098901098901099No Hit
GCCAAATGATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAA11.098901098901099No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGCA11.098901098901099Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGACCC11.098901098901099Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATATCGTATGCCGTC11.098901098901099No Hit
GTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGTT11.098901098901099No Hit
AAAGAAATCACAAAAGCAATTAAGAGAGCTCAAATACTGGGGTTTATGCCA11.098901098901099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.045.017
ACTGTAT50.045.032
GTATGCC50.045.042
ATCTCGT50.045.037
ATCTCCG50.045.010
CACATCT50.045.07
GCCCACG50.045.018
TATACAC50.045.03
AACTGTA50.045.031
TGCCGTC50.045.045
CAACTGT50.045.030
CCACGAG50.045.020
TATGCCG50.045.043
CATCTCC50.045.09
GTATCTC50.045.035
GAGCCCA50.045.016
TCAACTG50.045.029
CACGAGA50.045.021
CCCACGA50.045.019
ACATCTC50.045.08