FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_9.35100000044468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_9.35100000044468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26488
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC764428.858350951374206RNA PCR Primer, Index 45 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGT8323.1410450015101175No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC2140.8079130172153427RNA PCR Primer, Index 45 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG1880.7097553609181516No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT1650.6229235880398671No Hit
TATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTT630.23784355179704017RNA PCR Primer, Index 45 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.2227423739051646No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA580.2189670794321957No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA450.1698882512836001No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.16611295681063123No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.16611295681063123No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA410.15478707339172457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.15101177891875567No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.139685895499849No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1359106010268801No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCG350.1321353065539112No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC253.7880818E-545.045
TGCCGTC8000.040.7812545
ATGCCGT8850.037.11864544
CGTTCAT9000.036.7528
TTCATTA9000.036.7530
GTATGCC9000.036.7542
AATCTCG9000.036.7536
ACGTTCA9000.036.7527
CATTAAT9000.036.7532
TCTCGTA9000.036.7538
GACGTTC9000.036.7526
AGACGTT9000.036.7525
ACGAGAC9000.036.7522
ATTAATC9000.036.7533
CGTATGC9000.036.7541
TCGTATG9000.036.7540
CGAGACG9000.036.7523
TCATTAA9000.036.7531
GAGACGT9000.036.7524
GCCCACG8950.036.7039118
Produced by FastQC (version 0.11.2)