FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_89.35100000044e52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_89.35100000044e52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA43.2520325203252036No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC43.2520325203252036No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATC21.6260162601626018No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
GTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGA21.6260162601626018No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.6260162601626018No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTACAGATCCA10.8130081300813009Illumina PCR Primer Index 8 (96% over 27bp)
GCACCCGCGCCGAAGCCGAATGCACTGGGCCTGATCGTCAACGACATCCCA10.8130081300813009No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCT10.8130081300813009No Hit
CTTTTTTTTTTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATAC10.8130081300813009No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCCT10.8130081300813009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTT10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTGAAACCACCCCGTCC10.8130081300813009No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAACTGGTGGG10.8130081300813009RNA PCR Primer, Index 36 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGATACCCCCCTTACC10.8130081300813009No Hit
GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACGGGTGGTATCAACGC10.8130081300813009No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGT10.8130081300813009No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTACTGTCTCTTATACACATCTCCG10.8130081300813009No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAA10.8130081300813009No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.8130081300813009No Hit
GTGGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10.8130081300813009No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACCC10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCTTGTTCCCCCC10.8130081300813009No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCCTCTCGC10.8130081300813009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTCAACC10.8130081300813009No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCTG10.8130081300813009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTT10.8130081300813009No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTATTACCCCCCTTTCAC10.8130081300813009No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA10.8130081300813009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.8130081300813009No Hit
GTCCTGGTCGGATGCGGAACGGAGCAATCCGGTCCGCCGATCGATTCGGGG10.8130081300813009No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCCCCGTCG10.8130081300813009No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTA10.8130081300813009No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.8130081300813009No Hit
ACCGCGGGGAGATCGTCGATGCCTCGTCGGCCCAGCGATATGTCTGTCTCT10.8130081300813009No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACTCCCCAT10.8130081300813009Illumina PCR Primer Index 8 (96% over 27bp)
ACCCCGTACTCTGCGTTGATACCACTGCCCGTACCTGTCTCTTATACACAT10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA10.8130081300813009No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACACTTCTTTC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCACAAAATGCCTCCTCTACA10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCCCGTTCTC10.8130081300813009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAACCC10.8130081300813009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCCGTTAAACCCACC10.8130081300813009No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGC10.8130081300813009No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA10.8130081300813009No Hit
TTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTAT10.8130081300813009No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTATCCCCCCCCGT10.8130081300813009No Hit
GCCCAGAAGGCGTTCGAGCCGCTGGCCGCCCTGGCCGACGCCATCCTCTTC10.8130081300813009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTG10.8130081300813009No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACCCTGCG10.8130081300813009No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAATA10.8130081300813009RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAACAACAACATCTCTA10.8130081300813009RNA PCR Primer, Index 27 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.8130081300813009No Hit
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.8130081300813009No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA10.8130081300813009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCC10.8130081300813009No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGT10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.8130081300813009No Hit
GGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCG10.8130081300813009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTT10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACAGACGTCTTAGTCTCCCGTATGCCGTCC10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCTGCGTTT10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATACCACTGCTTTTTT10.8130081300813009No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAACCCCCGTCCTC10.8130081300813009No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCCC10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAACCCCCCC10.8130081300813009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.8130081300813009No Hit
CCCGTACTCTGCGTTGATACCACTGTCTCTTATACACATCTCCGAGCCCAC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAGAAAAAAAAAAAAAAA10.8130081300813009No Hit
GTACGGGGTGGTTCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACACAAAAA10.8130081300813009RNA PCR Primer, Index 27 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCCCGTA10.8130081300813009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAACCCCCGC10.8130081300813009No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAAAAGAGAAA10.8130081300813009Illumina PCR Primer Index 8 (96% over 27bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTATCCCCC10.8130081300813009No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCCC10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAAACCCCCCCGTT10.8130081300813009No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATACA10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACCACCCC10.8130081300813009No Hit
CAGTGGCATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGATATCTTC10.8130081300813009No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTGAACCCCC10.8130081300813009No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTACCCCCCCT10.8130081300813009No Hit
CCATAGGATCTTGGAAGGAGCTCTGAAATGGAAGGGACCTCTCTTTGGGTT10.8130081300813009No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAACCCCCTCCCCTTC10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATTCCCCCTTC10.8130081300813009No Hit
CGCCTGGACGTGCCGGTCTCCACCGGCAGCGTGCCTTTGCAGTCGGGGTAG10.8130081300813009No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC10.8130081300813009No Hit
GTAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTACCCCT10.8130081300813009No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.8130081300813009No Hit
GTTCCATGTGAACGGCACTTGCACATGGGCTGTCTCTTATACACATCTCCG10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATAC10.8130081300813009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTT10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTGAAACCACTCCCCCCT10.8130081300813009No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8130081300813009No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTAAAAAAAAAAAA10.8130081300813009No Hit
CTTATACACATCTCCGAGCCCACGACTGTTATACATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 39 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)