FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_88.35100000044e38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_88.35100000044e38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162735
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC3190419.60487909791993No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT51783.181860079269979No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG9770.6003625526162165No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT6680.4104833010722954No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3230.19848219497956798Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC35850.041.7344145
CCTTAGG250.0020991336.0093462
ATGCCGT41800.035.79374344
CTCCGAG43000.035.10878812
TCCGAGC43000.035.10878813
CCGAGCC43150.034.98673614
CCCACGA43050.034.96348619
CGTATGC43000.034.95181741
AGCCCAC43000.034.95181717
CGAGCCC43150.034.93459715
GCATGAT42900.034.92840232
CACGAGA43100.034.92292421
CCACGAG43100.034.92292420
ATCTCGT43050.034.91122437
TGATCTC42950.034.88773735
GAGCCCA43150.034.88245416
GCCCACG43100.034.87072418
TCTCGTA43100.034.87072438
TCGTATG43050.034.85896340
GATCTCG43000.034.8471736
Produced by FastQC (version 0.11.2)