FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_87.35100000044e1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_87.35100000044e1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184410
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC4207922.81817688845507No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT119316.469822677728973No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG12910.700070495092457No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT10310.5590803101784069No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3750.20335122824141857Illumina PCR Primer Index 1 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG2150.11658803752507998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG206.9933076E-445.032021
TGCCGTC46750.042.63734445
GCCGTCT2450.035.81265645
ATGCCGT59250.033.68009644
CGTATGC60650.032.86555541
TATGCCG60750.032.84848843
CTCGTAT60800.032.7844739
ATCTCGT60750.032.7744237
GTATGCC60750.032.7744242
GATCTCG60550.032.7712136
TCTCGTA60850.032.75753438
CCCACGA61100.032.73396319
GCCCACG61050.032.72392318
ACGAGAC61200.032.71723622
TCGTATG61000.032.71386340
GAGCCCA61150.032.70719516
CCACGAG61150.032.70719520
TCCGAGC61100.032.6971413
AGCCCAC61100.032.6971417
CGAGACT61100.032.6971423