FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_86.35100000044df3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_86.35100000044df3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134884
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC2601219.28471872127161No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT58104.30740488123128No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT8200.6079297766970138No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG7060.5234127101805996No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC2840.21055128851457547Illumina PCR Primer Index 1 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTT1530.11343079979834524Illumina PCR Primer Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG1350.10008599982206934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC27850.041.60442445
GACGTAT250.002101128735.99792542
CGAATTT250.002101128735.99792523
TACACTT250.002101128735.9979257
ATGCCGT34050.034.02887344
AGCCCAC35150.033.4760217
GAGCCCA35150.033.41201416
GCCCACG35100.033.3955118
CGAGCCC35350.033.35026615
TCCGAGC35450.033.31965613
CCGAGCC35400.033.30316514
CCCACGA35200.033.30063619
CCACGAG35250.033.253420
CACGAGA35350.033.22297721
GCCGTCT2100.033.2123745
GCATGAT35250.033.18957532
ACGAGAC35400.033.17605222
CTCCGAG35550.033.16264312
CGAGACT35350.033.15933223
AGCATGA35450.033.06579231
Produced by FastQC (version 0.11.2)