FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_85.35100000044dd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_85.35100000044dd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200937
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC8492542.264490860319405No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT195929.750319751962058No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCG32371.610952686662984No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCT9220.4588502864081777No Hit
ATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTCTTC3110.15477487968865863Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC90450.043.60407345
GCCGTCT2750.035.9976145
ATGCCGT111800.035.27717644
GCCCACG117050.034.1177718
TCCGAGC117500.034.08284413
AGCCCAC117350.034.06889317
CCGAGCC117550.034.06834814
GAGCCCA117500.034.04454816
CGAGCCC117500.034.04454815
CCACGAG117150.034.03103320
CACGAGA117050.034.02166421
CCCACGA117200.034.01651419
ACGAGAC117200.034.01651422
CTCCGAG117600.033.99646812
GAGACTA116950.033.97380424
CGAGACT117100.033.9687123
TCTCGTA116800.033.94038438
ATCTCGT116700.033.9309137
CTAAGCA116650.033.92616728
AGACTAA116850.033.9258625
Produced by FastQC (version 0.11.2)